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DyMIN STED + easy3D STED = unprecedented 3D STED resolution
A combination of expansion microscopy and DyMIN 3D STED reveals the ultrastructure of centrioles. Chlamydomonas reinhardtii centrioles immunostained with Abberior STAR RED for polyglutamylated tubulin. Samples by P. Guichard & D. Gambarotto (University of Geneva).
D. Gambarotto, F. U. Zwettler, M. Le Guennec, M. Schmidt-Cernohorska, D. Fortun, S. Borgers, J. Heine, J. G. Schloetel, M. Reuss, M. Unser, E. S. Boyden, M. Sauer, V. Hamel & P. Guichard, “Imaging cellular ultrastructures using expansion microscopy (U-ExM)”, Nature Methods (2019)
DyMIN STED is a co-development between Stefan Hell and coworkers and Abberior Instruments. Read all technical details in the current PNAS publication, 2017:
Nature review on DyMIN STED: G. Pacchioni, "Always look on the bright side of the fluorophore", Nature Review Materials (2017)